Fluorescence cryo-microscopy: current challenges and prospects
نویسندگان
چکیده
منابع مشابه
Fluorescence cryo-microscopy: current challenges and prospects
Studying biological structures with fine details does not only require a microscope with high resolution, but also a sample preparation process that preserves the structures in a near-native state. Live-cell imaging is restricted mostly to the field of light microscopy. For studies requiring much higher resolution, fast freezing techniques (vitrification) are successfully used to immobilize the...
متن کاملNeonatal Medicine in Iran: Current Challenges and Prospects
Background: Pediatric medicine in Iran has been known as a specialty for more than 50 years; nevertheless, the neonatal-perinatal medicine is still in the first decades of its life. Regarding this, the present study was conducted toprovide an overall view of the current situation of the neonatal medicine, its challenges, and prospects in Iran.Methods: For the purpose of the study, a questionnai...
متن کاملCryo-fluorescence microscopy facilitates correlations between light and cryo-electron microscopy and reduces the rate of photobleaching.
Fluorescence light microscopy (LM) has many advantages for the study of cell organization. Specimen preparation is easy and relatively inexpensive, and the use of appropriate tags gives scientists the ability to visualize specific proteins of interest. LM is, however, limited in resolution, so when one is interested in ultrastructure, one must turn to electron microscopy (EM), even though this ...
متن کاملAutoimmune hepatitis: current challenges and future prospects
Autoimmune hepatitis (AIH) is a chronic progressive liver disease characterized by high levels of aminotransferases and autoantibodies, hypergammaglobulinemia, and interface hepatitis. AIH affects all races and all ages worldwide, regardless of sex, although a preponderance of females is a constant finding. The etiology of AIH has not been completely elucidated, but immunogenetic background and...
متن کاملHepatic encephalopathy: current challenges and future prospects
Hepatic encephalopathy (HE) is a common complication of liver dysfunction, including acute liver failure and liver cirrhosis. HE presents as a spectrum of neuropsychiatric symptoms ranging from subtle fluctuating cognitive impairment to coma. It is a significant contributor of morbidity in patients with liver disease. HE is observed in acute liver failure, liver bypass procedures, for example, ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Current Opinion in Chemical Biology
سال: 2014
ISSN: 1367-5931
DOI: 10.1016/j.cbpa.2014.05.007